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KMID : 0665220180310030408
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Korean Journal of Food and Nutrition
2018 Volume.31 No. 3 p.408 ~ p.415
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Changes in γ-Aminobutyric Acid of Bitter Melon (Momordica charantia L.) with Different Cultivation Regions, Harvest Time and Maturation Stages, with Method Validation
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Lee Sang-Hoon
Jeong Yun-Sook
Song Jin
Hwang Kyung-A
Cho Soo-Muk
Hwang In-Guk
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Abstract
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This study aimed to investigate the changes in the γ-aminobutyric acid (GABA) content of bitter melon (Momordica charantia L.) cultivated from different regions, with different harvest times and at various maturation stages. Methods for observing the changes in GABA content were validated by determining the specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), and precision and accuracy using the HPLC-FLD system. Results showed high linearity in the calibration curve with a coefficient of correlation (R2) of 0.9999. The LOD and LOQ values for GABA were 0.29 and 0.87 μg/mL, respectively. The relative standard deviations for intra- and inter-day precision of GABA were less than 5%. The recovery rate of GABA was in the range of 98.77% to 100.50%. The average content of GABA was 0.93 mg/g and Cheongju showed highest GABA content of 1.88 mg/g. As the time of harvest increased from May to September, the GABA content decreased from 1.56 to 0.86 mg/g. Also, maturation of the bitter melon fruit was associated with a decreased in GABA content.
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KEYWORD
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bitter melon , Momordica charantia, γ-aminobutyric acid, method validation
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